Biofunctionalized all-polymer photonic lab on a chip with integrated solid-state light emitter

A photonic lab on a chip (PhLOC), comprising a solid-state light emitter (SSLE) aligned with a biofunctionalized optofluidic multiple internal reflection (MIR) system, is presented. The SSLE is obtained by filling a microfluidic structure with a phenyltrimethoxysilane (PhTMOS) aqueous sol solution containing a fluorophore organic dye. Its performance is assessed by measuring quinolone yellow, obtaining a limit of detection (LOD) of (0.60±0.01) µM. Finally, the MIR is selectively biofunctionalized with horseradish peroxidase (HRP) for the detection of hydrogen peroxide (H2O2) target analyte, obtaining a LOD of (0.7±0.1) µM for H2O2.
Light: Science & Applications (2015) 4, e271; DOI:10.1038/lsa.2015.44